This application is in the area of Chemical Biology (Area 2). Glycosylation,? which creates a diverse array of carbohydrate epitopes attached to cell surface? proteins and lipids, is an inherently complex system that is poorly understood.? Carbohydrates play crucial roles in a diverse array of medically relevant? biological processes from viral pathogenesis to tumor cell metastasis and stem? cell differentiation. However, due to the biosynthetic and molecular complexity of? these biopolymers, we have little comprehension of how glycan synthesis is? controlled. Systems-based approaches to biology, in which large datasets are? analyzed using bioinformatic algorithms, provide an important avenue for? exploring the mechanics of complex systems that cannot be predicted a-priori.? Application of such approaches to glycosylation however has been limited due to? the lack of methodology for high-throughput analysis of carbohydrates? (glycomics). Recent work in my laboratory on lectin microarray technology has? begun to address the analytical problems inherent in glycomics and thus pave? the way for systematic analysis of the glycome. I propose to use the NCI-60 cell? panel as a model system to integrate glycomic information with proteomic,? genomic and metabolic pertubation data to create a predictive model of how cell? surface glycosylation is encoded. To achieve this objective, we will reinvent? bioinformatics technology for glycomics including analytical and databasing? methods, integration of information and predictive modeling, providing useful? tools for the study of glycomics in a wide variety of contexts. Detailed knowledge? of how the genome and other factors control glycosylation will have a strong? impact on a diverse swath of fields where carbohydrates play important roles? including immunology, cancer research and developmental biology and may? impact their use as potential biomarkers for disease.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
NIH Director’s New Innovator Awards (DP2)
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Special Emphasis Panel (ZGM1-NDIA-G (01))
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Basavappa, Ravi
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New York University
Schools of Arts and Sciences
New York
United States
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