The objective of this fellowship application is to determine the gene expression profile in berberine-treated human cancer cells. An increasing amount of attention has been paid to the use of complementary and alternative medicine (CAM) as a part of the treatment for human cancer. A Chinese herb, Coptis chinensis, that is routinely used in China for treating gastroenteritis for thousands of years, may be developed as an anti-tumor agent. Coptis contains multiple constituents, and berberine is the major constituent. We have recently demonstrated that Coptis extract and berberine exhibit significant anti-proliferative activity in human cancer cells. However, the molecular mechanisms underlying Coptis or berberine-mediated anti-tumor activity are unknown. In this application, I propose to conduct a comprehensive gene expression profiling analysis in berberine-treated human cancer cells by focusing on three specific aims.
The first aim i s to conduct hybridizations of RNA samples prepared from berberine-treated human cancer cells.
The second aim i s to conduct comprehensive bioinformatic analyses on the microarray data set.
The third aim i s to verify potentially important targets of berberine using Northern blotting or quantitative RT-PCR. Taken together, by analyzing the expression profile of nearly 40,000 genes, I expect to identify a set of target genes that may function as intracellular mediators of berberine's anti-cancer action. My long-term goal is to elucidate the molecular bases of berberine-mediated antitumor activity, and to investigate the possible use of berberine and its derivatives as effective chemotherapy and/or chemopreventive agents in human cancer.
| Benítez, Almudena; González-Tejero, Marcos; Caballero, Álvaro et al. (2018) Almond Shell as a Microporous Carbon Source for Sustainable Cathodes in Lithium?Sulfur Batteries. Materials (Basel) 11: |
| Duan, Yan; Clemente-Juan, Juan M; Giménez-Saiz, Carlos et al. (2018) Large Magnetic Polyoxometalates Containing the Cobalt Cubane '[CoIIICo 3II (OH)3 Front Chem 6:231 |
| Ruiz Del Árbol, Nerea; Palacio, Irene; Otero-Irurueta, Gonzalo et al. (2018) On-Surface Bottom-Up Synthesis of Azine Derivatives Displaying Strong Acceptor Behavior. Angew Chem Int Ed Engl 57:8582-8586 |
| Ferrer, A; Cante, J C; Hernández, J A et al. (2018) Two-scale topology optimization in computational material design: An integrated approach. Int J Numer Methods Eng 114:232-254 |
| Davis, Meghan F; Ludwig, Shanna; Brigham, Emily P et al. (2018) Effect of home exposure to Staphylococcus aureus on asthma in adolescents. J Allergy Clin Immunol 141:402-405.e10 |
| Voto, Michele; Lopez-Diaz, Luis; Martinez, Eduardo (2017) Pinned domain wall oscillator as a tuneable direct current spin wave emitter. Sci Rep 7:13559 |
| Sawatzky, Richard; Laforest, Esther; Schick-Makaroff, Kara et al. (2017) Design and introduction of a quality of life assessment and practice support system: perspectives from palliative care settings. J Patient Rep Outcomes 2:36 |
| Gomez, A; Gich, M; Carretero-Genevrier, A et al. (2017) Piezo-generated charge mapping revealed through direct piezoelectric force microscopy. Nat Commun 8:1113 |
| Alejos, Oscar; Raposo, Víctor; Sanchez-Tejerina, Luis et al. (2017) Efficient and controlled domain wall nucleation for magnetic shift registers. Sci Rep 7:11909 |
| Abellán, Gonzalo; Ares, Pablo; Wild, Stefan et al. (2017) Noncovalent Functionalization and Charge Transfer in Antimonene. Angew Chem Int Ed Engl 56:14389-14394 |
Showing the most recent 10 out of 21 publications
