Millions of patients suffer from delayed wound healing stem from systemic diseases or external traumas, which can result in a loss of skin integrity, disability, and even death. An important aspect of healing is re- epithelialization that entails both proliferation and migration of keratinocytes at the periphery of the wound. When re-epithelialization is not achieved in a timely fashion, patients suffer from delayed wound healing. Understanding the role and function of keratinocytes in re-epithelialization will be of benefit to these patients. Oral wounds exhibit accelerated re-epithelialization when compared to equivalently sized skin wounds. Our laboratory recently identified a previously unnoticed landscape of non-coding RNA in saliva. Of particularly interests are three distinctive oral-piwi-interacting RNAs (oral-piRNAs) that are uniquely present in saliva and oral keratinocytes and appear to contribute to the oral wound re-epitheliazation phenotype. The applicant performed studies including in vitro scratch assays that demonstrated a continual increase of oral-piRNA expressions post wounding in human oral keratinocyte and not in skin keratinocytes. Furthermore, independent knockdown of these oral- piRNAs in the human oral wound scratch assay resulted in the significant delay and failure of oral wound closure. The overarching hypothesis of this proposal is that oral-piRNAs are intrinsic factors in human oral keratinocytes modulating the rapidity of wound closure. Based on the preliminary data, the applicant aims to validate the role of oral-piRNAs in oral wound healing in a 3D ex-vivo oral mucosal wound healing model in Aim 1 and then follow by elucidating the cellular and genetic factors responsible for the oral-piRNA mediated oral wound healing phenotypes, including epithelial migration and proliferation in Aim 2. By understanding oral-piRNA roles in normal oral wound healing, we can identify differences in skin wound healing regulation and alterations in delayed healing wounds. As we unravel how oral-piRNAs modulate human oral keratinocyte proliferation and migration, we may improve wound healing therapeutic developments. The long-term goals of this proposal are to advance the biological understanding and functions of piRNA relevant to human oral health and diseases and use the findings to benefit wound healing in patients. This F30 fellowship will prepare the trainee for an academic career to become an independent researcher in oral wound healing and piwi-interacting RNA biology.

Public Health Relevance

The proposed research will investigate the biological functions of oral-piRNA in relevance to oral wound healing by elucidating the cellular and genetic factors responsible for the oral-piRNA mediated oral wound healing phenotypes. Another major goal of this proposal is to identify and functionally characterize piRNA with respect to normal physiological functions in human somatic cells. The findings will contribute to public health by understanding why oral wounds exhibit accelerated healing compare to skin wounds.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Individual Predoctoral NRSA for M.D./Ph.D. Fellowships (ADAMHA) (F30)
Project #
5F30DE027615-04
Application #
10149158
Study Section
NIDR Special Grants Review Committee (DSR)
Program Officer
Frieden, Leslie A
Project Start
2017-09-01
Project End
2021-02-28
Budget Start
2020-09-01
Budget End
2021-02-28
Support Year
4
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Dentistry
Type
Schools of Dentistry/Oral Hygn
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095