Odor detection in mice is mediated by approximately 1000 different odorant receptors (ORs), which are expressed by olfactory sensory neurons (OSNs) in the olfactory epithelium (OE) of the nose. Each OSN expresses only one OR allele and OSNs with the same OR are scattered in the OE. However, their axons converge in a representative pair of glomeruli in the olfactory bulb (OB), producing a stereotyped map in which each glomerulus is dedicated to one OR. In addition to its role in recognizing odorants, there is growing evidence that the OR may also play a role in guiding the developing OSN axon to the correct target glomerulus in the OB. The evidence stems from OR gene swapping and deletion experiments and observations of OR protein in OSN axons. Is the axonal OR protein required for glomerular targeting? If so, what is the mechanism? We propose to investigate these questions. Using proteomic methods, we will identify proteins that are associated with ORs on axons versus cilia to explore the possible roles of axonal ORs. By doing so, we hope to better understand how axonal ORs may influence glomerular targeting.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Individual Predoctoral NRSA for M.D./Ph.D. Fellowships (ADAMHA) (F30)
Project #
5F30NS053055-03
Application #
7271959
Study Section
Special Emphasis Panel (ZNS1-SRB-M (18))
Program Officer
Riddle, Robert D
Project Start
2005-08-01
Project End
2009-07-31
Budget Start
2007-08-01
Budget End
2008-07-31
Support Year
3
Fiscal Year
2007
Total Cost
$32,698
Indirect Cost
Name
University of Washington
Department
Pathology
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195