Molecules involved in ligand-receptor interactions required for trypanosome entry into mammalian cells are prime targets for molecular intervention. A novel Trypanosoma cruzi gene, Tc-1, that codes for a 62 kDa protein has been identified and may be involved in parasite attachment and entry. The gene was recognized by a neutralizing monoclonal antibody, mAb 4A4 that recognizes epitopes on T. cruzi trypomastigote surface proteins. Our hypothesis is that Tc-1 codes for a novel 62 kDa protein that cardes an epitope required for trypanosome binding to mammalian cells. To test this hypothesis, the following specific aims were developed: (1) to characterize the Tc-1 gene coding for the 62 kDa protein, and (2) to determine the role of the 62 kDa protein in T. cruzi infection. Computer analysis, Northern, and Southern blots will be performed to obtain information about the possible function and developmental expression of Tc-l. The gene will be expressed and the 62 kDa protein pudfied; binding assays, immunoblots, and immunofluorescence studies will be done to try to localize the protein and determine its role in infection. The proposed studies will aid in the discovery of a novel T. cruzi ligand and advance our knowledge of the mechanisms involved in the eady molecular pathogenesis of T. cruzi infection.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31AI056674-01
Application #
6686127
Study Section
Special Emphasis Panel (ZRG1-MBC-1 (29))
Program Officer
Hernandez, Milton J
Project Start
2003-07-01
Project End
2005-06-30
Budget Start
2003-07-01
Budget End
2004-06-30
Support Year
1
Fiscal Year
2003
Total Cost
$37,789
Indirect Cost
Name
Meharry Medical College
Department
Microbiology/Immun/Virology
Type
Other Domestic Higher Education
DUNS #
041438185
City
Nashville
State
TN
Country
United States
Zip Code
37208