The ethyl acetate (EtOAc) extracts of three tropical fruits, Garcinia xanthochymus (Guttiferae), Mammea americana (Guttiferae) and Threobroma grandiflorum (Sterculia, displayed antioxidant activity in the americana (Guttiferae) and Theobroma grandiflorum (Sterculiaceae), displayed antioxidant activity in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant constituents of these extracts activity in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant constituents of these extracts have not been determined. In the proposed research, the three plants will be recollected from southern Florida., extracted with methanol, and separated using bioactivity (DPPH) guided reversed-phase high performance liquid chromatography-mass spectroscopy-mass spectroscopy. Isolated phytochemicals will be analyzed for their ability to prevent LDL-oxidation in vitro using a headscape GC bioassay. It is expected that this research will isolate known and novel antioxidants which inhibit LDL-oxidation in vitro. These compounds may serve as models for potential new therapeutic agents for the treatment of hypercholesterolemia.

Agency
National Institute of Health (NIH)
Institute
National Center for Complementary & Alternative Medicine (NCCAM)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31AT000062-02
Application #
6446852
Study Section
Special Emphasis Panel (ZAT1-B (03))
Program Officer
Hopp, Craig
Project Start
2001-09-01
Project End
Budget Start
2001-09-01
Budget End
2002-08-31
Support Year
2
Fiscal Year
2001
Total Cost
$23,351
Indirect Cost
Name
Herbert H. Lehman College
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
620128301
City
New York
State
NY
Country
United States
Zip Code
10468
Acuña, Ulyana Muñoz; Jancovski, Nikola; Kennelly, Edward J (2009) Polyisoprenylated benzophenones from Clusiaceae: potential drugs and lead compounds. Curr Top Med Chem 9:1560-80
Baggett, Scott; Protiva, Petr; Mazzola, Eugene P et al. (2005) Bioactive benzophenones from Garcinia xanthochymus fruits. J Nat Prod 68:354-60