The prognosis for primary malignant brain remains poor. In a Phase I trail, one specific type of effector cell, alloreactive cytotoxic T lymphocytes (CTL), have shown promise. It is important to understand how brain tumor cells acquire resistance to immune effector cells, such as alloreactive CTL. We hypothesize that models of immunotherapy resistant tumor will display stability of resistance as determine d by cytotoxicity assays. We hypothesize that immunoresistant populations will be less susceptible to cell death and will have an increased proliferative capacity relative to parental populations. Proliferation assays combined with the TUNEL assay and standard morphologic analysis will be used to compare immunoresistant and parental populations. Cytogenetic and gene expression profiles will help identify genes, proteins and chromosomal abnormalities that may provide brain tumor cells with immunity towards alloreactive CTL selective pressure.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31CA094834-01
Application #
6450499
Study Section
Special Emphasis Panel (ZRG1-RPHB-3 (20))
Program Officer
Bini, Alessandra M
Project Start
2001-09-06
Project End
Budget Start
2001-09-06
Budget End
2002-09-05
Support Year
1
Fiscal Year
2001
Total Cost
$21,682
Indirect Cost
Name
University of Colorado Denver
Department
Pathology
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045