The aim of this research is to contribute to the understanding of olfaction in the malaria vector mosquito Anopheles gambiae, especially as it pertains to host choice and vectorial capacity. Malaria continues to be a serious threat and demands focused investigation. The experiments outlined in this proposal seek to characterize one of the molecular components of olfaction in A. gambiae, an arrestin gene, AgArr1. As arrestins are known to desensitize heptahelical receptors (such as odorant receptors), these studies will initially investigate the role of arrestin genes in olfactory pathways using established mutants in the well-characterized Drosophila melanogaster model system. Behavioral and physiological paradigms including odor-baited traps and electroantennogram (EAG) recordings will be employed to identify and define olfactory phenotypes associated with the loss of insect arrestins. Subsequently, transgenic expression of AgArr1 in Drosophila will be performed to test the ability of the mosquito gene to compensate for these mutations and rescue the phenotype. In order to answer whether AgArr1 plays a similar role in mosquito olfactory pathways, double-stranded RNA interference gene silencing techniques will be utilized to decrease AgArr1 expression in A. gambiae for phenotypic analysis by EAG. These experiments will not only define olfactory deficits associated with loss of arrestin expression in insects, but also examine potential targets for antimalarial vector control.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31DC005264-01A1
Application #
6487803
Study Section
Communication Disorders Review Committee (CDRC)
Program Officer
Sklare, Dan
Project Start
2002-06-01
Project End
Budget Start
2002-06-01
Budget End
2003-05-31
Support Year
1
Fiscal Year
2002
Total Cost
$22,728
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Pharmacology
Type
Schools of Medicine
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Merrill, C Elaine; Sherertz, Tracy M; Walker, William B et al. (2005) Odorant-specific requirements for arrestin function in Drosophila olfaction. J Neurobiol 63:15-28
Merrill, C E; Pitts, R J; Zwiebel, L J (2003) Molecular characterization of arrestin family members in the malaria vector mosquito, Anopheles gambiae. Insect Mol Biol 12:641-50