The goal of this project is to understand how repeated experience can stably modify a neuron's activity by studying the molecules that affect the nuclear localization of the cGMP-dependent protein kinase EGL-4 during long-term odorant adaptation in C. elegans. This understanding will provide us with insight into mechanisms of learning and memory and molecular processes that underlie addictions. Nuclear localization of EGL-4 in odorant sensory neurons is necessary for the transition from short to long-term adaptation that occurs upon continual stimulation to an odorant. We propose to study the molecules that regulate this nuclear translocation. By utilizing a GFP-tagged EGL-4 molecule to visualize in real-time the behavior of EGL-4, we will examine the localization of EGL-4 in known components of both odorant chemotaxis and adaptation. We will also genetically screen for individuals that are defective in EGL-4 nuclear translocation to identify new components of adaptation that regulate the transition to long-term adaptation. The results from this study will give us a clearer understanding of neuronal plasticity. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31DC007031-03
Application #
7112920
Study Section
Communication Disorders Review Committee (CDRC)
Program Officer
Sklare, Dan
Project Start
2004-09-01
Project End
2007-08-31
Budget Start
2006-09-01
Budget End
2007-08-31
Support Year
3
Fiscal Year
2006
Total Cost
$30,655
Indirect Cost
Name
University of California Davis
Department
Neurosciences
Type
Schools of Medicine
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Lee, Jin I; O'Halloran, Damien M; Eastham-Anderson, Jeffery et al. (2010) Nuclear entry of a cGMP-dependent kinase converts transient into long-lasting olfactory adaptation. Proc Natl Acad Sci U S A 107:6016-21