There is unequivocal evidence from biochemistry and physiology that the diffusible second messenger 3'- 5'cyclic adenosine monophosphate (cAMP) is involved in taste transduction. In contrast, recent reports have emphasized the role of Ca2+ signaling in taste transduction (i.e. Receptor --> G protein --> PLCbeta2 --> delta[Ca2+]i). The significance of the original cAMP data is presently unresolved. The goal of my research is to re-investigate cAMP in taste transduction in the light of current understanding. I propose to image real-time changes in intracellular cAMP using novel, genetically-encoded cAMP reporters. These FRET-based reporters permit one to measure single-cell cAMP levels with excellent spatial and temporal resolution. This work will help define taste signaling at the single cell level and enhance our understanding of how gustatory information is processed and coded within taste buds. Specifically, I postulate that cAMP levels in subsets of individual taste receptor cells are altered in response to gustatory stimuli and that cAMP changes are mutually interactive with Ca2+ fluctuations.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31DC007591-01A2
Application #
7156456
Study Section
Communication Disorders Review Committee (CDRC)
Program Officer
Sklare, Dan
Project Start
2006-06-01
Project End
2008-05-31
Budget Start
2006-06-01
Budget End
2007-05-31
Support Year
1
Fiscal Year
2006
Total Cost
$34,134
Indirect Cost
Name
University of Miami School of Medicine
Department
Physiology
Type
Schools of Medicine
DUNS #
052780918
City
Miami
State
FL
Country
United States
Zip Code
33146
Roberts, Craig D; Dvoryanchikov, Gennady; Roper, Stephen D et al. (2009) Interaction between the second messengers cAMP and Ca2+ in mouse presynaptic taste cells. J Physiol 587:1657-68
Kim, Joung Woul; Roberts, Craig D; Berg, Stephanie A et al. (2008) Imaging cyclic AMP changes in pancreatic islets of transgenic reporter mice. PLoS One 3:e2127