Mr. Clemons proposes to determine the crystal structure of a small ribosomal protein, S15, from Bacillus stearothermophilus, to crystalize and solve the structure of the complex of S15 with its minimal binding site of 16S ribosomal RNA, and to crystalize initiation factor If2 alone and in complex with the initiator. He proposes to design a suitable rRNA as a target substrate for S15 binding. How this will achieve the same binding efficiency for s15 as native rRNA is not clearly described. The last goal of this proposal will be accomplished by a collaboration with Dr. U. RajBhandary from MIT, who will provide purified forymlmethionine charged initiator tRNA. The significance of studies that will be applied for understanding the biological functions of these proteins, is not described.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31GM019384-03
Application #
6125225
Study Section
Special Emphasis Panel (ZRG2-SSS-F (06))
Program Officer
Toliver, Adolphus
Project Start
1999-11-16
Project End
Budget Start
1999-11-16
Budget End
2000-11-15
Support Year
3
Fiscal Year
2000
Total Cost
$19,531
Indirect Cost
Name
University of Utah
Department
Biochemistry
Type
Schools of Medicine
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112