Vesicular transport is Saccharomyces cerevisiae is the interest of our lab. Previously, we identified a gene, SCD5, which encodes a novel protein with a post-Golgi secretory function. Little is known about the exact function of Scd5p. My research objectives are to further define the role of Scd5p in the secretory and endocytic pathways and to identify proteins that function in the same pathway as Scd5p using genetic approaches. A pseudoreversion screen using scd5-delta338, a temperature sensitive (ts) allele of SCD5, will be used to find gene products that interact genetically with Scd5p. In addition, a multicopy suppressor screen will be utilized as a secondary screen to identify proteins that interact with Scd5p or fulfill a similar function as Scd5p. After the isolation and cloning of the suppressors, gene disruptions will be analyzed for null phenotypes. Ultimately, mutant alleles of the suppressors will be tested for their effects on secretion, endocytosis, cell polarity, and cell morphology, which should assist in clarifying Scd5p's purpose within the cell.
| Chang, Ji Suk; Henry, Kenneth; Geli, Maria Isabel et al. (2006) Cortical recruitment and nuclear-cytoplasmic shuttling of Scd5p, a protein phosphatase-1-targeting protein involved in actin organization and endocytosis. Mol Biol Cell 17:251-62 |
| Henry, Kenneth R; D'Hondt, Kathleen; Chang, Ji Suk et al. (2003) The actin-regulating kinase Prk1p negatively regulates Scd5p, a suppressor of clathrin deficiency, in actin organization and endocytosis. Curr Biol 13:1564-9 |
| Henry, Kenneth R; D'Hondt, Kathleen; Chang, JiSuk et al. (2002) Scd5p and clathrin function are important for cortical actin organization, endocytosis, and localization of sla2p in yeast. Mol Biol Cell 13:2607-25 |
| Chang, Ji Suk; Henry, Kenneth; Wolf, Bianka L et al. (2002) Protein phosphatase-1 binding to scd5p is important for regulation of actin organization and endocytosis in yeast. J Biol Chem 277:48002-8 |
