The Herpes simplex virus (HSV) particle consists of a DNA-containing capsid surrounded by a lipid envelope which bears a diverse array of membrane proteins. Lining the inner surface of the envelope is a complex and poorly characterized layer of polypeptides termed tegument. The HSV envelope is acquired by budding of capsids through host cell organellar membranes, and envelope formation is essential for the subsequent transmission of viral disease. However, little is known of the molecular details of envelope assembly. A key step in envelope formation is likely to be the binding of tegument polypeptides to the cytoplasmic tails of viral envelope glycoproteins. This recruitment of tegument onto the membrane may then provide a binding site for the viral capsid. We have confirmed this hypothesis by developing assay systems which reconstitute the interaction of glycoprotein tails, tegument proteins and HSV capsids in vitro. With these assay systems available we now propose to use biochemical and recombinant DNA techniques to investigate the molecular events which take place during HSV particle assembly.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31GM020618-05
Application #
6891428
Study Section
Minority Programs Review Committee (MPRC)
Program Officer
Gaillard, Shawn R
Project Start
2002-05-28
Project End
2006-03-26
Budget Start
2005-05-28
Budget End
2006-03-26
Support Year
5
Fiscal Year
2005
Total Cost
$38,678
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461