Abstract

Tyrosyl-DNA phosphodiesterase I hydrolyzes Topoisomerase Ib from substrate DNA. Tyrosyl-DNA Phosphodiesterase appears to be involved in replication-induces double strand DNA breaks and an attractive chemotherapeutic target. This project aims to characterize this enzyme and discover an inhibitory molecule by deconvoluting combinatorial libraries.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31GM066372-01
Application #
6546202
Study Section
Special Emphasis Panel (ZRG1-F05 (29))
Program Officer
Toliver, Adolphus
Project Start
2002-08-15
Project End
Budget Start
2002-08-15
Budget End
2003-08-14
Support Year
1
Fiscal Year
2002
Total Cost
$24,974
Indirect Cost

  Institution

Name
San Diego State University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
073371346
City
San Diego
State
CA
Country
United States
Zip Code
92182

  Publications

Raymond, Amy C; Staker, Bart L; Burgin Jr, Alex B (2005) Substrate specificity of tyrosyl-DNA phosphodiesterase I (Tdp1). J Biol Chem 280:22029-35
Rideout, Marc C; Raymond, Amy C; Burgin Jr, Alex B (2004) Design and synthesis of fluorescent substrates for human tyrosyl-DNA phosphodiesterase I. Nucleic Acids Res 32:4657-64
Raymond, Amy C; Rideout, Marc C; Staker, Bart et al. (2004) Analysis of human tyrosyl-DNA phosphodiesterase I catalytic residues. J Mol Biol 338:895-906