The brevetoxins are neurotoxins produced by the dinofiagellate, Karenia brevis, which can cause serious illness in humans. People are exposed to brevetoxins through seafood consumption, dermal contact in water or breathing seaspray aerosols. The effects of these harmful toxins and how natural antagonists protect in their presence will be investigated to understand the nature of toxicity in fish and mammals. Since prior studies show that brevetoxins bind to orphan receptors on site 5 of voltage sensitive sodium channels, competition between toxin and potential antagonists will be examined. From receptor binding experiments in rat brain synaptosomes, the equilibrium disassociation constant, inhibition constant, and binding maximum of new compounds will be determined. Due to the recent increase and severity of toxic blooms worldwide, this project with its chemical and molecular approach to understanding toxic blooms, will have broad human and environmental impacts. Among the impacts this project will have on human health, results could guide the synthesis of possible therapeutics for human illness caused by toxins.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31GM069006-04
Application #
7082766
Study Section
Special Emphasis Panel (ZRG1-F10 (29))
Program Officer
Gaillard, Shawn R
Project Start
2003-08-18
Project End
2008-08-17
Budget Start
2006-08-18
Budget End
2007-08-17
Support Year
4
Fiscal Year
2006
Total Cost
$27,220
Indirect Cost
Name
University of North Carolina Wilmington
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
040036584
City
Wilmington
State
NC
Country
United States
Zip Code
28403
Gold, Elena P; Jacocks, Henry M; Bourdelais, Andrea J et al. (2013) Brevenal, a brevetoxin antagonist from Karenia brevis, binds to a previously unreported site on mammalian sodium channels. Harmful Algae 26:12-19