Abstract

The goal of this research is to understand the molecular interactions between rRNA and mRNA that are pertinent in selection in the A site.
Specific Aim 1 : Is the 16S ribosomal RNA triple mutant G530A, A1408C/G/L, and A1492G functionally comparable to wild type ribosomes? Specific Aim 2: What is the effect of interactions between C1054 and the base following UGA on release factor 2 selection. A literature survey of human genetic defects found that there are 179 unique nonsense mutations that result in disease (Atkinson, 1994). The effect of these mutations varies across a wide range including ovarian cancer, cystic fibrosis, and sex reversal. Understanding the mechanisms of selection will aid the development of drug and genetic therapies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31GM073575-01
Application #
6893538
Study Section
Special Emphasis Panel (ZRG1-F05 (29))
Program Officer
Gaillard, Shawn R
Project Start
2004-09-01
Project End
2006-08-31
Budget Start
2004-09-01
Budget End
2005-08-31
Support Year
1
Fiscal Year
2004
Total Cost
$26,645
Indirect Cost

  Institution

Name
University of Maryland Balt CO Campus
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
061364808
City
Baltimore
State
MD
Country
United States
Zip Code
21250