Basic fibroblast growth factor (bFGF) is a protein that stimulates cell migration and proliferation in wound healing. Recombinant bFGF is a promising therapeutic for diseases characterized by lack of angiogenesis, such as chronic wounds. In order for this protein to be bioactive in such harsh environments it has to be stabilized. Previous researchers have used heparin stabilization of other growth factors prior to controlled release. This work has proven successful, but heparin has strong bioactivity, is variable in activity and is difficult to modify. This proposal describes use of sulfated or sulfonated polymers such as poly(sulfonated styrene), to stabilize this growth factor. These polymers will be prepared in a controlled way by atom transfer radical polymerization (ATRP). It is expected that these materials will be useful for healing of chronic wounds.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31GM077086-01
Application #
7064630
Study Section
Special Emphasis Panel (ZRG1-GGG-G (29))
Program Officer
Gaillard, Shawn R
Project Start
2006-01-01
Project End
2009-09-30
Budget Start
2006-01-01
Budget End
2006-12-31
Support Year
1
Fiscal Year
2006
Total Cost
$29,588
Indirect Cost
Name
University of California Los Angeles
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Vazquez-Dorbatt, Vimary; Lee, Juneyoung; Lin, En-Wei et al. (2012) Synthesis of glycopolymers by controlled radical polymerization techniques and their applications. Chembiochem 13:2478-87
Vázquez-Dorbatt, Vimary; Tolstyka, Zachary P; Maynard, Heather D (2009) Synthesis of Aminooxy End-functionalized pNIPAAm by RAFT Polymerization for Protein and Polysaccharide Conjugation. Macromolecules 42:7650-7656
Vázquez-Dorbatt, Vimary; Tolstyka, Zachary P; Chang, Chien-Wen et al. (2009) Synthesis of a pyridyl disulfide end-functionalized glycopolymer for conjugation to biomolecules and patterning on gold surfaces. Biomacromolecules 10:2207-12
Christman, Karen L; Vazquez-Dorbatt, Vimary; Schopf, Eric et al. (2008) Nanoscale growth factor patterns by immobilization on a heparin-mimicking polymer. J Am Chem Soc 130:16585-91
Christman, Karen; Vayyzquez-Dorbatt, Vimary; Schopf, Eric et al. (2008) Nanoscale Growth Factor Patterns by Immobilization on a Heparin-Mimicking Polymer. J Am Chem Soc :