The objective of this proposal is to investigate the role of calcium (Ca) ions in initiation and maintenance of ventricular fibrillation (VF). This study will provide insight to scientists and clinicians about the mechanism of VF that leads to sudden cardiac death (SCD). Understanding the mechanism of VF is essential for developing new therapies and nursing interventions to prevent and to decrease the incidence of SCD.
The specific aims of this study are to: 1) measure intracellular membrane potential while recording the Ca transients; 2) determine the extent to which intracellular membrane potential and the Ca transients are correlated; 3) analyze the spatial distance between membrane potential and the Ca transients, and 4) develop an optical mapping system to permit simultaneous mapping of membrane potential and Ca transients. This study will use the right ventricle (RV) of nine swine during four modes of pacing including regular, incremental, irregular and rapid pacing resembling the rate of ventricular tachycardia and VF. Action potential duration restitution and Ca transients restitution will be constructed for each pacing modes. Pharmacological agents will be given, the effects will be documented and then the agents will be washed out and the effects will be documented again. At each stage of the study, electrical recording will be taken and optical mapping will be performed.

Agency
National Institute of Health (NIH)
Institute
National Institute of Nursing Research (NINR)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31NR007819-01A1
Application #
6486469
Study Section
National Institute of Nursing Research Initial Review Group (NRRC)
Program Officer
Armstrong, Nell
Project Start
2002-05-01
Project End
Budget Start
2002-05-01
Budget End
2003-04-30
Support Year
1
Fiscal Year
2002
Total Cost
$24,950
Indirect Cost
Name
University of California Los Angeles
Department
Type
Schools of Nursing
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Attin, Mina; Clusin, William T (2009) Basic concepts of optical mapping techniques in cardiac electrophysiology. Biol Res Nurs 11:195-207