Proper function of voltage gated potassium channels (Kv) is vital to health and disease, as demonstrated by identification of mutations in genes that encode Kv channel subunits as causing cardiac and neurological disorders in humans. Potassium (K) channels have key roles in the regulation of neuronal excitability. Over a hundred different subunits encoding distinct K channel subtypes have been identified so far. However, relating these many different channel subunits to the functional K currents observed in native neurons remains a major challenge. Thus, identifying the native composition of macromolecular potassium channel complexes is a critical step to understand the regulation and function of native K currents. Kv2.1 is a major subunit encoding delayed rectifier potassium currents in neurons. Recently, Kv2.1 has been proposed to be involved in neuronal apoptosis. The objective of this proposal is biochemical purification of the native Kv2.1 channel multi-protein complex and the analysis of the molecular components of the Kv2.1 channel complex by proteomic approach. Completion of the proposed research will identify Kv2.1-associated proteins, which provides insights into the mechanism of current diversity and the regulation of neuronal delayed rectifier potassium currents.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31NS050991-01
Application #
6885182
Study Section
Special Emphasis Panel (ZRG1-F03B (20))
Program Officer
Stewart, Randall R
Project Start
2004-09-30
Project End
2006-08-31
Budget Start
2004-09-30
Budget End
2005-08-31
Support Year
1
Fiscal Year
2004
Total Cost
$43,543
Indirect Cost
Name
Johns Hopkins University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218