Alcohol is among the most widely abused drugs in the world, yet our understanding of the mechanisms by which it regulates brain function and behavior remain largely unclear. This proposal describes the identification of a mutation (P-16-99) in a Drosophila Discs Large (Dig) gene, which causes an altered behavioral response to ethanol. The goal of this project is to use PI 6-99 to investigate the role of Dig in the development of ethanol tolerance.
The specific aims are: ? Aim #1: To obtain phenotypic rescue of the tolerance defect of the PI6-99 strain using transgenic flies expressing the dig gene and to define the precise molecular lesion induced by the PI6-99 mutation. ? Aim #2. To determine the temporal requirements for dig function during ethanol tolerance development. ? Aim #3. To define the structural domains of Dig that play roles in ethanol tolerance development. ? Aim #4: To study the role of known Dig-interacting proteins, such as the NMDA receptor and CaM Kinase II in ethanol tolerance. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32AA015470-03
Application #
7109337
Study Section
Special Emphasis Panel (ZAA1-EE (20))
Program Officer
Neuhold, Lisa
Project Start
2004-09-09
Project End
2007-10-08
Budget Start
2006-09-09
Budget End
2007-10-08
Support Year
3
Fiscal Year
2006
Total Cost
$48,796
Indirect Cost
Name
University of California San Francisco
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Maiya, Rajani; Lee, Seonok; Berger, Karen H et al. (2012) DlgS97/SAP97, a neuronal isoform of discs large, regulates ethanol tolerance. PLoS One 7:e48967
Wolf, Fred W; Eddison, Mark; Lee, Seonok et al. (2007) GSK-3/Shaggy regulates olfactory habituation in Drosophila. Proc Natl Acad Sci U S A 104:4653-7