The goal of this proposal to study the genetic variability of the human immunodeficiency virus type l (HIV-l), the causative agent of the acquired immunodeficiency syndrome (AIDS). Specifically, we are interested in the temporal relationship and sequence variability between the integrated proviral DNA and viral RNA during HIV-1 infection and the phenotypic consequences of genome variation of HIV-1 under selective pressure.
Our specific aims are to examine: l) the temporal variations in proviral DNA in peripheral blood monocyte cells (PBMC) and viral RNA during active HIV-1 infection; 2) the evolution of proviral DNA and plasma viral RNA sequences during antiretroviral therapy to suppress HIV-1 replication; and 3) the replicative fitness of zidovudine and lamivudine-resistant mutant HIV-1 as compared to that of wild type HIV- 1. The proposed research will further our understanding of population dynamics of cells infected with HIV-1 as well as the genetic variability of HIV-1 and its phenotypic consequences, particularly during antiretroviral therapy, and may aid in designing effective antiretroviral therapy regimens to treat AIDS.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32AI010578-03
Application #
6533985
Study Section
Special Emphasis Panel (ZRG1-AARR-3 (01))
Program Officer
Bradac, James A
Project Start
2002-08-20
Project End
Budget Start
2002-08-20
Budget End
2003-03-19
Support Year
3
Fiscal Year
2002
Total Cost
$31,526
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599