Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32AI054007-03
Application #
6833545
Study Section
Special Emphasis Panel (ZRG1-F04 (20))
Program Officer
Korpela, Jukka K
Project Start
2003-01-01
Project End
2005-07-31
Budget Start
2005-01-01
Budget End
2005-07-31
Support Year
3
Fiscal Year
2005
Total Cost
$33,912
Indirect Cost
Name
Harvard University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115
Gomez Garcia, Inmaculada; Stevenson, Clare E M; Uson, Isabel et al. (2010) The crystal structure of the novobiocin biosynthetic enzyme NovP: the first representative structure for the TylF O-methyltransferase superfamily. J Mol Biol 395:390-407
Gomez Garcia, Inmaculada; Freel Meyers, Caren L; Walsh, Christopher T et al. (2008) Crystallization and preliminary X-ray analysis of the O-carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides. Acta Crystallogr Sect F Struct Biol Cryst Commun 64:1000-2
Stevenson, Clare E M; Freel Meyers, Caren L; Walsh, Christopher T et al. (2007) Crystallization and preliminary X-ray analysis of the O-methyltransferase NovP from the novobiocin-biosynthetic cluster of Streptomyces spheroides. Acta Crystallogr Sect F Struct Biol Cryst Commun 63:236-8
Jakimowicz, Piotr; Tello, Monica; Meyers, Caren L Freel et al. (2006) The 1.6-A resolution crystal structure of NovW: a 4-keto-6-deoxy sugar epimerase from the novobiocin biosynthetic gene cluster of Streptomyces spheroides. Proteins 63:261-5
Tello, Monica; Jakimowicz, Piotr; Errey, James C et al. (2006) Characterisation of Streptomyces spheroides NovW and revision of its functional assignment to a dTDP-6-deoxy-D-xylo-4-hexulose 3-epimerase. Chem Commun (Camb) :1079-81
Pacholec, Michelle; Freel Meyers, Caren L; Oberthur, Markus et al. (2005) Characterization of the aminocoumarin ligase SimL from the simocyclinone pathway and tandem incubation with NovM,P,N from the novobiocin pathway. Biochemistry 44:4949-56
Freel Meyers, Caren L; Oberthur, Markus; Xu, Hui et al. (2004) Characterization of NovP and NovN: completion of novobiocin biosynthesis by sequential tailoring of the noviosyl ring. Angew Chem Int Ed Engl 43:67-70
Pi, Na; Meyers, Caren L Freel; Pacholec, Michelle et al. (2004) Mass spectrometric characterization of a three-enzyme tandem reaction for assembly and modification of the novobiocin skeleton. Proc Natl Acad Sci U S A 101:10036-41
Freel Meyers, Caren L; Oberthur, Markus; Heide, Lutz et al. (2004) Assembly of dimeric variants of coumermycins by tandem action of the four biosynthetic enzymes CouL, CouM, CouP, and NovN. Biochemistry 43:15022-36
Jakimowicz, Piotr; Freel Meyers, Caren L; Walsh, Christopher T et al. (2003) Crystallization and preliminary X-ray studies on the putative dTDP sugar epimerase NovW from the novobiocin biosynthetic cluster of Streptomyces spheroides. Acta Crystallogr D Biol Crystallogr 59:1507-9