The overall goal of this proposal is directed at understanding virus and receptor interactions. Three- dimensional structures will be determined of small non-enveloped viruses interacting with cellular components that modulate the viral life cycle during cell recognition, entry or viral uncoating. Recognition of specific cellular receptors is a significant part of the determination of host range and tissue tropism. Viruses have evolved to use a large variety of cell-surface molecules as receptors with some viruses using commonly found chemical groups whereas other viruses recognize very specific molecules. Hence, the distribution of the receptor will determine the tropism of the virus and the symptoms of the infection. For the research proposed here, two different viral systems will be used: positive stranded ssRNA picornaviruses, including echovirus 7 and coxsackieB3; also the ssDNA-containing parvoviruses, including family members feline panleukopenia virus and canine parvovirus. Both families of viruses are simple, non-enveloped, icosahedral viruses with an external diameter of approximately 300 Angstroms.
| Plevka, Pavel; Hafenstein, Susan; Harris, Katherine G et al. (2010) Interaction of decay-accelerating factor with echovirus 7. J Virol 84:12665-74 |
| Cotmore, Susan F; Hafenstein, Susan; Tattersall, Peter (2010) Depletion of virion-associated divalent cations induces parvovirus minute virus of mice to eject its genome in a 3'-to-5' direction from an otherwise intact viral particle. J Virol 84:1945-56 |
| Hafenstein, Susan; Bowman, Valorie D; Sun, Tao et al. (2009) Structural comparison of different antibodies interacting with parvovirus capsids. J Virol 83:5556-66 |
| Hafenstein, Susan; Bowman, Valorie D; Chipman, Paul R et al. (2007) Interaction of decay-accelerating factor with coxsackievirus B3. J Virol 81:12927-35 |
| Hafenstein, Susan; Palermo, Laura M; Kostyuchenko, Victor A et al. (2007) Asymmetric binding of transferrin receptor to parvovirus capsids. Proc Natl Acad Sci U S A 104:6585-9 |
