The long-term objectives of the research proposal is to elucidate the mechanism of radiation-induced DNA strand scission. Despite the number of experimental studies on this topic to date, key mechanistic details in the DNA cleavage process are still a point of contention. Though a rigorous theoretical study of intermediates formed under radiative conditions, the relative efficacy of these species as DNA damaging agents and their preferred mode of attack on the deoxyribose moiety of DNA (the direct precursor to strand breakage) will be determined. This study will provide keen mechanistic insight into an area of extreme biomedical significance, as radiation-induced DNA lesions lead ultimately to cell death. In addition, such radiative processes are also used for the treatment of cancer and other diseases.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32CA076770-02
Application #
2896313
Study Section
Special Emphasis Panel (ZRG2-SSS-1 (02))
Program Officer
Lohrey, Nancy
Project Start
1999-04-01
Project End
Budget Start
1999-04-01
Budget End
2000-03-31
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Houk, K N; Hietbrink, Bruce N; Bartberger, Michael D et al. (2003) Nitroxyl disulfides, novel intermediates in transnitrosation reactions. J Am Chem Soc 125:6972-6
Bartberger, Michael D; Liu, Wei; Ford, Eleonora et al. (2002) The reduction potential of nitric oxide (NO) and its importance to NO biochemistry. Proc Natl Acad Sci U S A 99:10958-63