The objective of this research proposal is to characterize the oncogenes p3k and akt, and to provide a molecular explanation for the remarkable tissue specificity of tumor induced by P3k and Akt overexpression. This study will generate information on the mechanisms by which P3k and Akt induce cell transformation and tumors in chicken, and provide new insights into the mechanisms of angiogenesis during tumorigenesis.
The specific aims of the proposal are: 1. Study the role of P3k and Akt in angiogenesis in vivo. The experiments will determine whether P3k and Akt induce angiogenesis, and study the mechanism of the angiogenesis induced by P3k and Akt. 2. Determine the transforming properties of P3k and Akt in specific cell types. The studies will determine whether P3k and Akt overexpression can affect specific cell types, and provide the explanation of the tumor specificity induced by P3k and Akt. 3. Test whether P3k and Akt mediate the expression of vascular endothelial growth factor (VEGF) and other angiogenic factors. The experiments will determine whether P3k and Akt can mediate VEGF expression, study whether the function of VEGF is required for P3k-and Akt-induced angiogenesis, test whether P3k and Akt affect the expression of other angiogenic factors, and study the signal transduction pathway of the P3k-and Akt- induced angiogenesis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32CA077892-01A1
Application #
2772472
Study Section
Special Emphasis Panel (ZRG2-SSS-1 (01))
Program Officer
Lohrey, Nancy
Project Start
1999-08-06
Project End
Budget Start
1999-02-02
Budget End
2000-02-01
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037