Cancer Immunotherapy Through Intratumoral Activation of Recall Responses The overarching goal for this project is to elucidate whether recall antigen expression in the tumor microenvironment can be used to enhance cancer immunotherapy, or potentially, serve as a new anti-cancer modality. These studies will also determine whether immunological memory/pre- existing immunity against an oncolytic poliovirus, PVSRIPO, is an impediment or asset to therapy. PVSRIPO is showing remarkable promise in patients with glioblastoma, recently received Breakthrough Therapy Designation from the FDA, and represents a unique approach delivering a potent human recall antigen (polio capsid) into human tumors. Surmounting evidence indicates that recall response activation leads to both innate and adaptive inflammation, capable of inducing immune responses against new antigens. This proposal seeks to harness the power of these responses to enhance cancer immunotherapy. This will be accomplished through testing the role of pre-existing anti-polio immunity on PVSRIPO therapy in mouse models of melanoma and brain cancer in affecting both efficacy and antitumor immunity. Additionally, polio and tetanus antigens, which are recall antigens for nearly everyone in the world, will be exogenously delivered into murine melanoma and brain tumors to test if intratumoral inflammation and engagement of antitumor immunity occurs in mice with recall responses to these antigens. Lastly, an in vitro human system will be used to evaluate the effects of recall antigen induced inflammation on antigen presenting cells and priming of new T cells. These studies aim to exhaustively test the adjuvant potential of recall responses in the context of cancer immunotherapy; which has remained an unexplored route to engage antitumor immunity to date.

Public Health Relevance

The generation of new antitumor T cell immunity is an eminent objective to deliver better cancer immunotherapy to patients. Activation of recall responses through the intratumoral delivery of recall antigens may facilitate antitumor T cell activity and could serve as a new cancer immunotherapy approach. The delivery of oncolytic polio, which is currently showing promise in clinical trials for glioblastoma multiforme (GBM), could accomplish such activation in patients.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32CA224593-01
Application #
9464141
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Jakowlew, Sonia B
Project Start
2018-01-18
Project End
2021-01-17
Budget Start
2018-01-18
Budget End
2019-01-17
Support Year
1
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Duke University
Department
Neurosurgery
Type
Schools of Medicine
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
Thompson, Eric M; Brown, Michael; Dobrikova, Elena et al. (2018) Poliovirus Receptor (CD155) Expression in Pediatric Brain Tumors Mediates Oncolysis of Medulloblastoma and Pleomorphic Xanthoastrocytoma. J Neuropathol Exp Neurol 77:696-702
Walton, Ross W; Brown, Michael C; Sacco, Matthew T et al. (2018) Engineered Oncolytic Poliovirus PVSRIPO Subverts MDA5-Dependent Innate Immune Responses in Cancer Cells. J Virol 92: