The goal of this project is to find the specific interaction of the agonist DPDPE in the human delta opioid receptor. This will be accomlished through that incoporation of a photoaffinity labled amino acid, 4-azidotrafluorphylalanine, into DPDPE. After formation of the DPDPE analopgues, they will be allowed to interact with the opioid receptor nd bindinf affinities will be obtained. The photoaffinity labled DPDPE will covalently link to the receptor using a pulsed laer or a 200W arc-lamp. The bound DPDPE/receptor will then solubized and purified using affinity chromatography. This will then be using trysin and chymothrypsin and the fragments will be analyzed via mass spectroscopy to determine where the DPDPE bound to the recetor. Once this is accomplished, site specific mutagenisis will be done on the human delta opioid receptor in order to determine how binding affinities are affected.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32DA005787-01A1
Application #
2520974
Study Section
Human Development Research Subcommittee (NIDA)
Project Start
1998-03-19
Project End
Budget Start
1997-09-20
Budget End
1998-09-19
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Arizona
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
City
Tucson
State
AZ
Country
United States
Zip Code
85721