The specific aims of the research in this proposal are to identify and characterize genes whose expression patterns are influenced by acute LSD administration in the brains of rats. Candidate genes will be identified from a pool of clones isolated in previous Differential Display PCR experiments. Differential expression within the brain for candidates will be determined and characterized by RNase protection and tissue in situ experiments. Antibodies will be generated to analyze candidate protein expression. Differential expression will be tested after administration of different hallucinogens. The receptor mediated pathways in which the differentially expressed genes act will be determined in experiments employing a variety of receptor antagonists. Ultimately these genes, their products and the pathways that are involved may be investigated for their potential role in animal behavior and in human mental disorders such as schizophrenia. This training and research could provide the basis for future work in establishing an independent research program in an academic environment.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32DA005993-02
Application #
6362806
Study Section
Human Development Research Subcommittee (NIDA)
Program Officer
Babecki, Beth
Project Start
2001-03-01
Project End
Budget Start
2001-03-01
Budget End
2002-02-28
Support Year
2
Fiscal Year
2001
Total Cost
$42,772
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Pharmacology
Type
Schools of Medicine
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212