? Some very exciting and important pathophysiological roles for the anandamide/cannabinoid receptor signaling system have been identified. Cannabinoid receptor activation by anandamide has been implicated in hemorrhagic hypotension, endotoxin-induced septic shock, pain perception, neuroprotection, and the alleviation of spasticity in a variety of conditions. The anandamide/cannabinoid receptor signaling pathway is important for normal embryonic fertilization, implantation, and development. The purpose of the present research is to provide for a better understanding of the biochemistry of anandamide inactivation through an uptake carrier. ? ? This will be accomplished by: (a) Measuring the accumulation of anandamide in a model devoid of intracellular binding sites and fatty acid amide hydrolase (FAAH); (b) Determining under what conditions, cell types, and to what extent a coupling between anandamide uptake and FAAH activity occurs; and (c) Isolating, purifying, and sequencing an intracellular anandamide binding protein. ? ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32DA016510-03
Application #
6917932
Study Section
Human Development Research Subcommittee (NIDA)
Program Officer
Babecki, Beth
Project Start
2003-07-01
Project End
2005-12-01
Budget Start
2005-07-01
Budget End
2005-12-01
Support Year
3
Fiscal Year
2005
Total Cost
$17,643
Indirect Cost
Name
Medical College of Wisconsin
Department
Pharmacology
Type
Schools of Medicine
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
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