Adolescent marijuana use is a prominent public health concern. Indeed, exposure to marijuana during adolescence is correlated with drug abuse and addiction in adulthood and predicts future cocaine abuse. Changing national attitudes on marijuana use and the resultant push for the reform of marijuana laws (23 states and the District of Columbia currently have legalized marijuana in some form) highlight a need to examine the long-term neurobiological consequences of cannabinoid exposure. I have collected preliminary data showing that adolescent exposure to a cannabinoid agonist disrupts the experience of cocaine reward in adulthood and results in cocaine-induced conditioned place aversion. These data confirm the work of others showing that cannabinoid exposure in adolescence disturbs normal brain development and produces lasting effects on behavior. In this study, we intend to utilize cutting-edge electrochemical and optogenetic techniques to investigate the effects of cannabinoid exposure in adolescence on cocaine experience and reinforcement in adulthood.
Specific aim 1 utilizes pharmacological manipulations to determine if cannabinoid exposure in adolescence exerts its effects on adult cocaine experience through actions at CB1 receptors. In the same animals we will employ the electrochemical technique fast-scan cyclic voltammetry to measure whether cannabinoid exposure during adolescence changes subsecond dopamine release evoked by cocaine and cocaine-associated contextual cues and assess how cannabinoid exposure affects the initiation and escalation of cocaine self-administration.
Specific aim 2 uses optogenetic tools to test whether cannabinoids alter cocaine experience through disruption of dopaminergic function. Altogether these experiments will provide novel information concerning the neural mechanisms through which cannabinoid exposure in adolescence disturbs brain development and influences cocaine reward and reinforcement in adulthood.

Public Health Relevance

According to the NIDA-funded 2014 Monitoring the Future Study, approximately 34-45% of high school students have used cannabis at least once, with 5.8% of high school seniors reporting daily marijuana use. Importantly, a growing body of literature links adolescent cannabinoid use with an increased incidence of drug addiction in adulthood. The proposed study will investigate how cannabinoid use during adolescence affects the neural mechanisms underlying the subjective effects of cocaine and cocaine reinforcement.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32DA039690-02
Application #
9251139
Study Section
Special Emphasis Panel (ZRG1-F02A-J (20)L)
Program Officer
Babecki, Beth
Project Start
2016-03-09
Project End
2018-03-08
Budget Start
2017-03-09
Budget End
2018-03-08
Support Year
2
Fiscal Year
2017
Total Cost
$59,166
Indirect Cost
Name
University of Maryland Baltimore
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
188435911
City
Baltimore
State
MD
Country
United States
Zip Code
21201