The goal of this proposal is to identify the genes that specify the pancreatic fates to isolate and characterize putative stem cells from the pancreas. We will use differential cloning techniques to isolate genes that are specifically expressed in an undifferentiated but committed pancreas rudiment. In situ hybridization will be used to verify temporal and spatial expression pattern of candidate genes. We will explore the use of retroviruses to introduce antisense RNA of candidate genes into pancreatic epithelium or ectopicaly express them in other cell types and test their effects. In addition, we will establish pancreatic ductile cell lines and study their stem cell capability to form pancreatic islet and acinar cells. These tests will be performed both in vivo and in vitro: the cell lines will be grown in organ culture system to test their capacity to form islet and acinar cells. Cell lines will also be implanted into the embryonic and postnatal pancreas and their ability to form islet and acinar cells be determined by immuno-staining.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32DK009832-03
Application #
6362968
Study Section
Biological Sciences 2 (BIOL)
Program Officer
Podskalny, Judith M,
Project Start
2001-03-01
Project End
Budget Start
2001-03-01
Budget End
2002-02-28
Support Year
3
Fiscal Year
2001
Total Cost
$41,996
Indirect Cost
Name
Harvard University
Department
Microbiology/Immun/Virology
Type
Schools of Arts and Sciences
DUNS #
071723621
City
Cambridge
State
MA
Country
United States
Zip Code
02138
Gu, Guoqiang; Dubauskaite, Jolanta; Melton, Douglas A (2002) Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. Development 129:2447-57