The main objective of this proposal is to characterize the mechanism of insulin gene transcription in pancreatic beta cells. In particular, I want to define the role of ERK (extracellular signal-regulated kinase) in this process.
The specific aims of the project are: 1) to identify and characterize direct and indirect substrates of activated ERK that are linked to insulin gene transcription; 2) to determine the localization of ERK activation of specific substrates and transcription factors involved in insulin gene transcription; and 3) to characterize the effects of various stimuli (glucose, free fatty acids, insulin, cAMP). Ultimately, these studies will result in a better understanding of beta cell function and insulin signaling in relation to diabetes mellitus.
| Ferrer, Jennifer R; Chokechanachaisakul, Attasit; Wertheim, Jason A (2015) New Tools in Experimental Cellular Therapy for the Treatment of Liver Diseases. Curr Transplant Rep 2:202-210 |
| Uzarski, Joseph S; Su, Jimmy; Xie, Yan et al. (2015) Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development. J Vis Exp :e53271 |
| Uzarski, Joseph S; Bijonowski, Brent M; Wang, Bo et al. (2015) Dual-Purpose Bioreactors to Monitor Noninvasive Physical and Biochemical Markers of Kidney and Liver Scaffold Recellularization. Tissue Eng Part C Methods 21:1032-43 |
