Abstract

Light-mediated signal transduction in vertebrate retina involves the absorption of light by the chromophore of the visual pigment rhodopsin. Absorption of a photon leads to the isomerization of the chromophore, formation of activated rhodopsin, and subsequent phosphorylation of rhodopsin by rhodopsin kinase. Arrestin preferentially binds light- activated, phosphorylated rhodopsin rather than nonphosphorylated rhodopsin, thus quenching light-mediated signal transduction. It is hypothesis using recombinant arrestin constructs in which the two positively charged domains are either eliminated, substituted with negatively charged amino acids, or substituted with neutral amino acids. These constructs will be expressed in a heterologous system and the binding of these expressed arrestin proteins to phosphorylated rhodopsin will be compared to that of native arrestin.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32EY006641-01
Application #
2160694
Study Section
Visual Sciences C Study Section (VISC)
Project Start
1995-08-15
Project End
Budget Start
1995-02-15
Budget End
1996-02-14
Support Year
1
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
University of Florida
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
073130411
City
Gainesville
State
FL
Country
United States
Zip Code
32611

  Publications

Du, Jianhai; An, Jie; Linton, Jonathan D et al. (2018) How Excessive cGMP Impacts Metabolic Proteins in Retinas at the Onset of Degeneration. Adv Exp Med Biol 1074:289-295
Zhu, Siyan; Yam, Michelle; Wang, Yekai et al. (2018) Impact of euthanasia, dissection and postmortem delay on metabolic profile in mouse retina and RPE/choroid. Exp Eye Res 174:113-120
Chao, Jennifer R; Knight, Kaitlen; Engel, Abbi L et al. (2017) Human retinal pigment epithelial cells prefer proline as a nutrient and transport metabolic intermediates to the retinal side. J Biol Chem 292:12895-12905
Kanow, Mark A; Giarmarco, Michelle M; Jankowski, Connor Sr et al. (2017) Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye. Elife 6:
Du, Jianhai; Rountree, Austin; Cleghorn, Whitney M et al. (2016) Phototransduction Influences Metabolic Flux and Nucleotide Metabolism in Mouse Retina. J Biol Chem 291:4698-710
Du, Jianhai; Yanagida, Aya; Knight, Kaitlen et al. (2016) Reductive carboxylation is a major metabolic pathway in the retinal pigment epithelium. Proc Natl Acad Sci U S A 113:14710-14715
Contreras, Laura; Ramirez, Laura; Du, Jianhai et al. (2016) Deficient glucose and glutamine metabolism in Aralar/AGC1/Slc25a12 knockout mice contributes to altered visual function. Mol Vis 22:1198-1212
Du, Jianhai; Linton, Jonathan D; Hurley, James B (2015) Probing Metabolism in the Intact Retina Using Stable Isotope Tracers. Methods Enzymol 561:149-70
Hurley, James B; Chao, Jennifer R (2015) It's never too late to save a photoreceptor. J Clin Invest 125:3424-6
Adijanto, Jeffrey; Du, Jianhai; Moffat, Cynthia et al. (2014) The retinal pigment epithelium utilizes fatty acids for ketogenesis. J Biol Chem 289:20570-82

Showing the most recent 10 out of 13 publications