Abstract

(From the Applicant?s Abstract): A novel mouse model of retinal degeneration, the 5A11/Basigin null mouse, has recently been characterized by this laboratory and others. 5A11/Basigin is a membrane glycoprotein expressed on the apical and basal surfaces of the retinal pigmented epithelium, Muller glial cells, and photoreceptor cells (cell body and inner segments) of the mouse retina. Inactivation of the mouse 5A11/Basigin gene results in abnormal maturation of photoreceptor outer segments that precedes the eventual degeneration of photoreceptors, with a complete loss of inner and outer segments. Understanding the molecular mechanisms through which this specific degeneration occurs will undoubtedly lead to insights relevant to the clinical treatment of retinal dystrophies, the major cause of blindness in the Western world. Therefore, the studies proposed herein are aimed at investigating the molecular diversity of 5A11/Basigin gene expression during mouse retinal development as a path to understanding the function of this molecule.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32EY013918-02
Application #
6555832
Study Section
Special Emphasis Panel (ZRG1-SSS-R (20))
Program Officer
Mariani, Andrew P
Project Start
2002-09-30
Project End
Budget Start
2002-09-30
Budget End
2003-09-29
Support Year
2
Fiscal Year
2002
Total Cost
$48,148
Indirect Cost

  Institution

Name
University of Florida
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
073130411
City
Gainesville
State
FL
Country
United States
Zip Code
32611

  Publications

Tokar, Derek; van Ekeris, Leslie; Linser, Paul J et al. (2017) Characterization of the Expression of Basigin Gene Products Within the Pineal Gland of Mice. Cell Mol Neurobiol 37:1141-1145
Pablo, Kristine Anne V; Ochrietor, Judith D (2013) Deletion of the Basigin gene results in reduced mitochondria in the neural retina. Biochem Biophys Res Commun 438:546-50
Ochrietor, Judith D; Moroz, Tatiana P; Linser, Paul J (2010) The 2M6 antigen is a Muller cell-specific intracellular membrane-associated protein of the sarcolemmal-membrane-associated protein family and is also TopAP. Mol Vis 16:961-9
Finch, NiCole A; Linser, Paul J; Ochrietor, Judith D (2009) Hydrophobic interactions stabilize the basigin-MCT1 complex. Protein J 28:362-8
Ochrietor, Judith D; Clamp, Michael F; Moroz, Tatiana P et al. (2005) Carbonic anhydrase XIV identified as the membrane CA in mouse retina: strong expression in Muller cells and the RPE. Exp Eye Res 81:492-500
Clamp, Michael F; Ochrietor, Judith D; Moroz, Tatiana P et al. (2004) Developmental analyses of 5A11/Basigin, 5A11/Basigin-2 and their putative binding partner MCT1 in the mouse eye. Exp Eye Res 78:777-89
Ochrietor, Judith D; Linser, Paul J (2004) 5A11/Basigin gene products are necessary for proper maturation and function of the retina. Dev Neurosci 26:380-7
Philp, Nancy J; Ochrietor, Judith D; Rudoy, Carla et al. (2003) Loss of MCT1, MCT3, and MCT4 expression in the retinal pigment epithelium and neural retina of the 5A11/basigin-null mouse. Invest Ophthalmol Vis Sci 44:1305-11
Ochrietor, Judith D; Moroz, Tatiana P; van Ekeris, Leslie et al. (2003) Retina-specific expression of 5A11/Basigin-2, a member of the immunoglobulin gene superfamily. Invest Ophthalmol Vis Sci 44:4086-96