Incorporation of metallated nucleosides into DNA at fixed and known locations is crucial to examination of electron transfer rates and the variation of these rates with structure, distance, and position. A new synthetic strategy has been developed for covalent attachment of ruthenium acceptor and donor complexes to duplex DNA through solid phase synthesis. Three series of metallated duplexes are proposed in which to investigate the effect of 1) donor-acceptor distance, 2) site of donor or acceptor covalent attachment, and 3) driving force on the rates of electron transfer in DNA. The rates will be measured by transient absorption spectroscopy, and distance dependence and driving force parameters will be obtained through data manipulation. The insight gained from these studies will allow determination of structural parameters of electron transfer in DNA.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32GM020016-01
Application #
2862881
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1999-03-29
Project End
Budget Start
1999-03-29
Budget End
2000-03-28
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost
Name
California Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
078731668
City
Pasadena
State
CA
Country
United States
Zip Code
91125