We will synthesize a small library of heptapeptide scaffolds to explore the effects of scaffold conformation on cyclization and cell permeability. We will select several novel cell-permeable cyclic scaffolds for elaboration into libraries of potential protein ligands and screen them for effects on the cell cycle in a number of different assays. Several active compounds will be chosen for further study. We are particularly interested in compounds that arrest cells in mitosis by directly perturbing the degradation of cyclin B or by targeting components of the mitotic spindle and thus triggering the mitotic checkpoint pathway.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM020037-02
Application #
6179893
Study Section
Biological Sciences 2 (BIOL)
Program Officer
Marino, Pamela
Project Start
2000-07-01
Project End
Budget Start
2000-07-01
Budget End
2001-06-30
Support Year
2
Fiscal Year
2000
Total Cost
$37,516
Indirect Cost
Name
Harvard University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115
Peterson, J R; Lokey, R S; Mitchison, T J et al. (2001) A chemical inhibitor of N-WASP reveals a new mechanism for targeting protein interactions. Proc Natl Acad Sci U S A 98:10624-9