Histone mRNAs are tightly regulated during the mammalian cell cycle. Most of the regulation is post-transcriptional, mediated by the 3' end of histone mRNA. The protein that binds to the 3 end of histone mRNA, the stem-loop binding protein SLBP, is a likely candidate to mediate the post-transcriptional regulation of histone mRNA. The levels of SLBP are also tightly regulated during the mammalian cell cycle. The signals regulating histone mRNA metabolism are likely to be important in the regulation of cell growth, and hence be possible targets for novel chemotherapy approaches. The mechanisms by which histone mRNA expression is coupled to the known regulators of the cell cycle are not understood. This proposal will elucidate the molecular mechanisms by which SLBP is regulated, focusing on regions in the protein, which regulate the half-life of histone mRNA and on the sequences in SLBP mRNA, which regulate its translation.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM020151-02
Application #
6342757
Study Section
Biological Sciences 2 (BIOL)
Program Officer
Wolfe, Paul B
Project Start
2000-01-01
Project End
Budget Start
2001-01-01
Budget End
2001-12-31
Support Year
2
Fiscal Year
2001
Total Cost
$34,832
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Biochemistry
Type
Schools of Medicine
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
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Moore, Jennifer C; Sumerel, Jan L; Schnackenberg, Bradley J et al. (2002) Cyclin D and cdk4 are required for normal development beyond the blastula stage in sea urchin embryos. Mol Cell Biol 22:4863-75
Schnackenberg, Bradley J; Marzluff, William F (2002) Novel localization and possible functions of cyclin E in early sea urchin development. J Cell Sci 115:113-21
Sumerel, J L; Moore, J C; Schnackenberg, B J et al. (2001) Cyclin E and its associated cdk activity do not cycle during early embryogenesis of the sea Urchin. Dev Biol 234:425-40