Abstract

The sequence specific recognition of DNA by proteins is extremely important for many biological processes. Due to its importance new motifs which sequence specifically recognize DNA are useful in the design of therapeutics as well as in the construction of new proteins. This proposal outlines the design of a novel motif for the sequence specific recognition of DNA and its subsequent use in the design of a DNA dependent peptide ligase. This motif consists of a heterodimeric coiled coil in which one of the solvent exposed faces is design to recognize the major groove of the AP-1 site.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM020333-02
Application #
6363194
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Program Officer
Marino, Pamela
Project Start
2001-03-01
Project End
Budget Start
2001-03-01
Budget End
2002-02-26
Support Year
2
Fiscal Year
2001
Total Cost
$34,501
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037