Cotranslational targeting and insertion of proteins into the protein conducting channel (PCC) of the endoplasmic reticulum (ER) requires the action of three interacting GTPases; SRP54, and the SRP receptor heterodimer SRalpha, and SRbeta. The SRbeta is one component of this pathway for which little is known. The proposed research will use a combination of biochemical analysis and X-ray crystallography to characterize the function and the structure of SRbeta alone and as a unit of the SRP receptor. The identification of regions involved in the interactions and transmission of signals will serve as a major tool in elucidating the mechanisms by which the combination of GTPases regulates protein translocation.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM020520-02
Application #
6385179
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Tompkins, Laurie
Project Start
2000-04-01
Project End
Budget Start
2001-04-01
Budget End
2002-03-31
Support Year
2
Fiscal Year
2001
Total Cost
$40,196
Indirect Cost
Name
Rockefeller University
Department
Anatomy/Cell Biology
Type
Other Domestic Higher Education
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065