The trafficking of macromolecules between the nucleus and cytoplasm is tightly regulated. Molecules must be first brought to the nuclear pore complex (NPC) by a set of specific adapter proteins. The steps that follow as the complex translocates through the NPC are poorly understood. To further understand these processes, I will utilize a novel in vitro system to look at transport through single NPCs. The advantage of this system is the access to single NPCs This allows for the reconstitution of transport using isolated factors. Initially, this system will be used to address basic questions regarding the poorly understood export of ribosomal subunits and the signal recognition particle. This system will also be applied to answering questions regarding the adapters and the proteins that compose the NPC itself.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32GM020655-01
Application #
6208519
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Flicker, Paula F
Project Start
2000-09-30
Project End
Budget Start
2000-09-30
Budget End
2001-09-29
Support Year
1
Fiscal Year
2000
Total Cost
$30,916
Indirect Cost
Name
Rockefeller University
Department
Anatomy/Cell Biology
Type
Other Domestic Higher Education
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065