DNA polymerase beta (Pol-beta) functions in base excision repair and meiosis. The high error rate of Pol-beta suggests that this polymerase might contribute to mutations, resulting in human disease. This application proposes to establish a tissue-specific knockout mouse to study the role of Pol-beta in meiosis. This research will use the Cre/loxP recombination system to direct testes-specific deletion of pol-beta. The function of Pol-beta in meiosis will be defined by histological and immunohistological examination of Pol-beta deletion mutants. In addition, a lambda phage-based mouse mutagenesis assay will be used to determine the impact of pol-beta inactivation on mutational frequency during meiosis. Finally, the yeast two-hybrid system will be used to identify proteins that interact with Pol-beta in meiosis. The proposed research will contribute to our understanding of the function of Pol-beta in vivo, as well as broaden our knowledge of meiosis.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32GM020720-01
Application #
6210802
Study Section
Biological Sciences 2 (BIOL)
Program Officer
Tompkins, Laurie
Project Start
2000-07-01
Project End
Budget Start
2000-07-01
Budget End
2001-06-30
Support Year
1
Fiscal Year
2000
Total Cost
$32,416
Indirect Cost
Name
Yale University
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520