The purpose of this proposal is to understand how maternal factors are unequally localized in the embryos and thereby defining the polarities of the embryo and the fates of the blastomeres in C. elegans. During early C. elegans embryogenesis, PIE-1 protein is localized exclusively to germline blastomeres. In this proposal we will identify factors that regulate unequal localization of PIE-1 in the C. elegans embryo. These factors will help us to build molecular models on the mechanism of cell polarity and germ cell specification. In a preliminary study, we have already identified two very intriguing proteins, ALP-1 and UBC-9, which suggested that translational regulation and post-translational modification may be involved in this process. More importantly, PI3-1, ALP-1 and UBC-9 all have homologues in other organisms, suggesting that there may be conserved mechanism in generating asymmetry in cells that leads to asymmetric division. To understand the mechanism of asymmetric division in which two daughter cells with different developmental potentials are produced is of basic fundamental importance in developmental biology. This research is also relevant to human development and fertility as well as to mechanisms of stem-cell renewal and oncogenesis.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM020795-02
Application #
6518887
Study Section
Biological Sciences 2 (BIOL)
Program Officer
Wolfe, Paul B
Project Start
2001-05-01
Project End
Budget Start
2002-05-01
Budget End
2003-04-30
Support Year
2
Fiscal Year
2002
Total Cost
$48,148
Indirect Cost
Name
University of Massachusetts Medical School Worcester
Department
Other Basic Sciences
Type
Schools of Medicine
DUNS #
660735098
City
Worcester
State
MA
Country
United States
Zip Code
01655