18. GOALS FOR FELLOWSHIP TRAINING AND CAREER NAME (Last, first, middle initial) Chen, Joseph C. INSTITUTION MENTOR Harvey Mudd College Nancy V. Hamlett Harvard University Jonathan Beckwith INSTITUTION/COMPANY SUPERVISO_EMPLOYER AstraZeneca Ed Buurman The proposed fellowship will deepen my training as a research scientist. It will also enhance my understanding of how cellular and developmental events are regulated in microorganisms. I will learn to combine tools and concepts from different disciplines--such as genetics, biochemistry, bioinformatics, and cell biology--to study the integration of regulatory signals in a bacterial cell. After completing the fellowship, I plan to continue research on the molecular mechanisms that control cellular differentiation and cell cycle progression. This postdoctoral training will prepare me for a career as an independent investigator. 19. NAME AND DEGREE(S) Lucille Shapiro, Ph.D. 20. POSITION/RANK Professor of Developmental Biology 21. RESEARCH INTERESTS/AREAS development, cell cycle control and regulation, localization of proteins and chromosomes it_1:_1i1_,_m: u:(.m.l-v=_ 22. DESCRIPTION (Do not exceed space provided) The bacterium Caulobacter crescentus coordinates cell cycle progression and cellular differentiation to follow a precise developmental program. To ensure faithful execution of this program, the bacterium employs multiple regulatory strategies, a key one being proteolysis of specific proteins at defined times and locations. This proposal aims to determine the roles of membrane-bound metalloproteases in the regulation of developmental events. Many of these events in C. crescentus involve dynamically positioned transmembrane proteins, and integral membrane proteases may influence the localization or stability of such proteins. An assessment will be made of whether and how select metalloproteases participate in cell cycle regulation. Their proteolytic activities and targets will also be analyzed. Understanding how proteolysis controls critical cellular processes in this model organism will help elucidate how similar mechanisms operate during normal cell proliferation in humans. PHS 416-1 (Rev. 12/98) Form Page 2 BB cc Individual NRSA Application Table of Contents ========================================Section End===========================================
| Curtis, Patrick D; Quardokus, Ellen M; Lawler, Melanie L et al. (2012) The scaffolding and signalling functions of a localization factor impact polar development. Mol Microbiol 84:712-35 |
| Fields, Alexander T; Navarrete, Charlene S; Zare, Alaa Ziad et al. (2012) The conserved polarity factor podJ1 impacts multiple cell envelope-associated functions in Sinorhizobium meliloti. Mol Microbiol 84:892-920 |
| Arellano, Benjamin H; Ortiz, Janett D; Manzano, Janet et al. (2010) Identification of a dehydrogenase required for lactose metabolism in Caulobacter crescentus. Appl Environ Microbiol 76:3004-14 |
