The goal of my research proposal is to understand the molecular mechanisms underlying the transmembrane translocation of solutes by the Major Facilitator Superfamily (MFS) of transporters. MFS transporters are the largest family of transporters classified to date, and includes at least 28 subfamilies and several thousand proteins. These transporters can translocate an extraordinary variety of solutes, yet are still proposed to share a common evolutionary background and structural architecture. The primary technique that will be employed for my research will be that of x-ray crystallography. I intend to improve my chances of success in this project by using many prokaryotic homologs for crystallization trials. Ten prokaryotic homologs have already been expressed to high levels, and preliminary crystals have been grown for several of these homologs. Crystallographic analysis of any one MFS transporter will reveal insight into the function of this entire class of important proteins. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32GM068304-01
Application #
6646199
Study Section
Special Emphasis Panel (ZRG1-F04 (20))
Program Officer
Cassatt, James
Project Start
2003-07-10
Project End
2006-01-09
Budget Start
2003-07-10
Budget End
2004-07-09
Support Year
1
Fiscal Year
2003
Total Cost
$42,648
Indirect Cost
Name
U of L Imperial Col of Sci/Technlgy/Med
Department
Type
DUNS #
227092590
City
London
State
Country
United Kingdom
Zip Code
SW7 2-AZ
Iverson, Tina M (2006) Evolution and unique bioenergetic mechanisms in oxygenic photosynthesis. Curr Opin Chem Biol 10:91-100
Ferreira, Kristina N; Iverson, Tina M; Maghlaoui, Karim et al. (2004) Architecture of the photosynthetic oxygen-evolving center. Science 303:1831-8