A bioinformatics-driven molecular genetics approach will be used to study the role of alternative splicing as a gene regulation mechanism in plant hormone signaling using the model plant Arabidopsis thaliana. High-density oligonucleotide microarrays will be used to examine the effects of exogenous auxin, brassinolide, and gibberellic acid treatments on (i) the expression of Arabidopsis genes that encode putative RNA-binding protein splicing factors; and (2) pre-mRNA splicing. T-DNA mutant insertion lines will be identified for the genes encoding hormone-regulated putative RNA-binding proteins and assayed for hormone associated phenotypic changes. Putative hormone-regulated alternative splice variants detected using the Affymetrix arrays will be further analyzed using RT-PCR, denaturing high performance liquid chromatography (DHPLC) and final confirmation by sequencing. Genes confirmed to exhibit hormone-regulated alternative splicing will be selected for use in developing an alternative splicing reporter gene system that will be tested experimentally in transgenic plants.
| Guo, Feng; Wan, Liling; Zheng, Aiping et al. (2014) Structural insights into the tumor-promoting function of the MTDH-SND1 complex. Cell Rep 8:1704-1713 |
| Koussevitzky, Shai; Nott, Ajit; Mockler, Todd C et al. (2007) Signals from chloroplasts converge to regulate nuclear gene expression. Science 316:715-9 |
| Mockler, Todd C; Chan, Simon; Sundaresan, Ambika et al. (2005) Applications of DNA tiling arrays for whole-genome analysis. Genomics 85:1-15 |
| Nemhauser, Jennifer L; Mockler, Todd C; Chory, Joanne (2004) Interdependency of brassinosteroid and auxin signaling in Arabidopsis. PLoS Biol 2:E258 |
| Mockler, Todd C; Yu, Xuhong; Shalitin, Dror et al. (2004) Regulation of flowering time in Arabidopsis by K homology domain proteins. Proc Natl Acad Sci U S A 101:12759-64 |
