Unnatural amino acids will be incorporated in vivo at specific positions within G-protein coupled receptors in order to fluorescently label proteins and detect and characterize protein/protein and protein/ligand interactions. Specific labeling of a single amino acid within a protein with a fluorescent label will provide information about the local microenvironment of that residue. Labeling with a photocrosslinking amino acid, followed by shotgun proteomics by the Biological Mass Spectrometry Lab at TSRI, will identify ligand interactions of that residue in human orphan receptors expressed in yeast. Photocrosslinking using an unnatural amino acid incorporated in C. elegans will be used to characterize a pathway involved with aging. Work with C. elegans will be in collaboration with Andrew Dillin at The Salk Institute.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM071280-02
Application #
6878087
Study Section
Special Emphasis Panel (ZRG1-F04 (20))
Program Officer
Lograsso, Philip
Project Start
2004-04-01
Project End
2006-01-31
Budget Start
2005-04-01
Budget End
2006-01-31
Support Year
2
Fiscal Year
2005
Total Cost
$41,330
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037