Rap1p, an essential DNA binding protein of Saccharomyces cerevisiae plays roles in silencing large stretches of Chromatin and in the activation and repression of hundreds of genes. In rapidly growing yeast cells, many of the genes regulated by Rap1p are among the most highly transcribed in the genome; however, during other growth conditions, including meiosis, many of the same genes are repressed. Understanding how a single transcription factor can mediate different transcriptional responses based on cell type, stage of development or growth conditions is a fundamental biological problem. The experiments described in this proposal are designed to identify factors that allow Rap1p to evoke different transcriptional outcomes during meiosis as compared to vegetatively growing cells. First, the distribution of Rap1 p and its affects on patterns of expression in vegetative growth and throughout meiosis will be mapped using ChlP-chip and expression microarray experiments. We will then compare the patterns of distribution under each condition and correlate binding patterns with gene expression. Finally, using sensitive reporter assay systems, we will perform screens to identify and characterize cofactors, competing transcription factors and protein modifiers that are required for modulating the activity of Rap1p during meiosis.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32GM073341-01A1
Application #
6998736
Study Section
Special Emphasis Panel (ZRG1-F08 (20))
Program Officer
Portnoy, Matthew
Project Start
2005-09-01
Project End
2007-08-31
Budget Start
2005-09-01
Budget End
2006-08-31
Support Year
1
Fiscal Year
2005
Total Cost
$48,296
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599