The objective of this proposal is to understand the mechanism of chromatin assembly, especially regarding how this process is coupled to DNA synthesis. Proper chromatin assembly is important for the transfer of epigenetic marks from mother to daughter cells and also for maintaining the integrity of the organism's genome. This proposal will focus on determining the mechanism of chromatin assembly facilitated by the histone H3/H4 chaperone Chromatin Assembly Factor 1 (CAF-1) using a combination of reconstituted DNA replication assays as well as biochemical and biophysical characterization of the CAF-1 complex. The stoichiometry of CAF-1 mediated histone incorporation will be investigated during replication-coupled chromatin assembly. Replication coupled assays will be developed to identify accessory proteins and reaction intermediates during replication coupled chromatin assembly. Additionally non-replication based Asf1-dependent CAF-1 chromatin assembly will be investigated using biochemical assays. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM076863-02
Application #
7188557
Study Section
Special Emphasis Panel (ZRG1-F08 (20))
Program Officer
Haynes, Susan R
Project Start
2006-03-01
Project End
2009-02-28
Budget Start
2007-03-01
Budget End
2008-02-29
Support Year
2
Fiscal Year
2007
Total Cost
$48,796
Indirect Cost
Name
University of Massachusetts Medical School Worcester
Department
Genetics
Type
Schools of Medicine
DUNS #
603847393
City
Worcester
State
MA
Country
United States
Zip Code
01655
Campeau, Eric; Ruhl, Victoria E; Rodier, Francis et al. (2009) A versatile viral system for expression and depletion of proteins in mammalian cells. PLoS One 4:e6529