Abstract

This project will develop the next generation of LSPR sensors based on plasmon resonance of metallic nanoprism arrays. LSPR sensors, invented and developed in the Van Duyne lab, enable high sensitivity measurement of molecular concentration and in situ characterization of binding constants. Recently LSPR sensors measured the concentration of """"""""amyloid derived diffusable ligands"""""""" (ADDLs), a promising biomarker for and possible cause of Alzheimer's disesase, in the cerebral spinal fluid of Alzheimer's and aging controls; preliminary results showed higher levels in Alzheimer's patents and further tests are in progress. This project enhances current techniques by 1) Optimizing nanoprism shape and chemistry, and modulating binding by changing pH, temperature, and ionic strength. 2) Depositing nanoprisms onto flexible and stretchable surfaces to modulate nanoprism spacing and electromagnetic field coupling. 3) Interfacing the LSPR substrates with mass spectroscopy (MS) instruments for complimentary binding studies and MS identification. These sensors hold promise for study of ADDL chemistry, as well as for Alzheimer's diagnosis and continuing evaluation of treatment. The techniques may also be used for other biomarkers and diseases. ? ?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM077020-02
Application #
7161313
Study Section
Special Emphasis Panel (ZRG1-F04A (20))
Program Officer
Fabian, Miles
Project Start
2005-12-05
Project End
2008-12-04
Budget Start
2006-12-05
Budget End
2007-12-04
Support Year
2
Fiscal Year
2007
Total Cost
$48,796
Indirect Cost

  Institution

Name
Northwestern University at Chicago
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
160079455
City
Evanston
State
IL
Country
United States
Zip Code
60201

  Publications

Hall, W Paige; Modica, Justin; Anker, Jeffrey et al. (2011) A conformation- and ion-sensitive plasmonic biosensor. Nano Lett 11:1098-105
Bingham, Julia M; Anker, Jeffrey N; Kreno, Lauren E et al. (2010) Gas sensing with high-resolution localized surface plasmon resonance spectroscopy. J Am Chem Soc 132:17358-9
Biggs, Kevin B; Camden, Jon P; Anker, Jeffrey N et al. (2009) Surface-enhanced Raman spectroscopy of benzenethiol adsorbed from the gas phase onto silver film over nanosphere surfaces: determination of the sticking probability and detection limit time. J Phys Chem A 113:4581-6
Anker, Jeffrey N; Hall, W Paige; Lambert, Mary P et al. (2009) Detection and Identification of Bioanalytes with High Resolution LSPR Spectroscopy and MALDI Mass Spectrometry. J Phys Chem C Nanomater Interfaces 113:5891-5894
Anker, Jeffrey N; Hall, W Paige; Lyandres, Olga et al. (2008) Biosensing with plasmonic nanosensors. Nat Mater 7:442-53
Hall, W Paige; Anker, Jeffrey N; Lin, Yao et al. (2008) A calcium-modulated plasmonic switch. J Am Chem Soc 130:5836-7