Host-microbe interactions, both beneficial and pathogenic, are a rapidly developing area of research with important implications for understanding the normal microbiota, as well as its disturbance by infection. The monospecific symbiosis between the bioluminescent bacteria Vibrio fischeri and the Hawaiian bobtail squid, Euprymna scolopes, provides us with an ideal model with which to study the earliest events of symbiotic initiation. This proposal will focus on a key bacterial signaling pathway, chemotaxis, which allows a bacterium to recognize and respond to changes in its environment. Characterization of bacterial chemotaxis in the context of a beneficial host-microbe association will provide a more complete picture of how microbes interact with and respond to the complex environments presented by host tissues, while migrating from the ambient seawater into a host organism. The overall goals of this research are to characterize the methyl-accepting chemotaxis proteins (MCPs) which act as the ligand receptors in the bacterial chemotaxis machinery, as well as the regulation of these receptors. MCPs have been well studied to date in E. coli. However, E. coli only uses a set of five of these receptors, while the majority of bacteri are known to have many more. V. fischeri is predicted to encode 43 MCPs, and their expression is known to be relevant to colonization of the host. The colonization of E. scolopes by V. fischeri has been shown to occur in several distinct stages, and involves the bacterium migrating through several environments and tissues as it moves from the bacterioplankton to the interior light organ of the animal. This research will further clarify the specific ligands recognized by V. fischeri, and which of these are involved during the initiation of symbiosis.
The first aim will address the receptor-ligand interactions, and will be pursued using established techniques such as soft-agar motility assays and capillary-tube migration assays, as well as a FRET-based assay that allows investigation of specific ligand-protein interactions in a receptorless- background strain of E. coli.
The second aim will investigate the regulation of these MCPs, and will utilize both transcriptional profiling by RNAseq and fluorescent reporter methods, in experiments designed to determine whether distinct stages of the colonization involve specific receptors. The tools used and developed during the course of this project will also be applicable to other studies, both in the Ruby lab and in other labs studying host-microbe interactions. This project will also provide significant training to the post-doctoral fellow in several areas, includng advanced microscopy and transcriptional profiling techniques.

Public Health Relevance

Beneficial host-microbe interactions are rapidly emerging as a newly recognized essential component of human health. By studying and understanding how these associations begin in model organisms, broad implications to the complex microbiota of mammalian systems can be inferred. The project I propose here focuses on the regulation of a bacterial behavior during the establishment of a beneficial, monospecific relationship between Vibrio fischeri and Euprymna scolopes, in order to better characterize the signaling that occurs during the early stages of a bacterial-host association.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Postdoctoral Individual National Research Service Award (F32)
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Special Emphasis Panel (ZRG1)
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Reddy, Michael K
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University of Wisconsin Madison
Schools of Medicine
United States
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Pan, Shu; Nikolakakis, Kiel; Adamczyk, Paul A et al. (2017) Model-enabled gene search (MEGS) allows fast and direct discovery of enzymatic and transport gene functions in the marine bacterium Vibrio fischeri. J Biol Chem 292:10250-10261
Thompson, Luke R; Nikolakakis, Kiel; Pan, Shu et al. (2017) Transcriptional characterization of Vibrio fischeri during colonization of juvenile Euprymna scolopes. Environ Microbiol 19:1845-1856
Nikolakakis, K; Monfils, K; Moriano-Gutierrez, S et al. (2016) Characterization of the Vibrio fischeri Fatty Acid Chemoreceptors, VfcB and VfcB2. Appl Environ Microbiol 82:696-704
Nikolakakis, K; Lehnert, E; McFall-Ngai, M J et al. (2015) Use of Hybridization Chain Reaction-Fluorescent In Situ Hybridization To Track Gene Expression by Both Partners during Initiation of Symbiosis. Appl Environ Microbiol 81:4728-35